The Advanced Photon Source
a U.S. Department of Energy Office of Science User Facility

Boosting Lead-Contaminated Soil Microbial Health with Biochar

Lead contamination in soil poses a serious threat to human health, especially for children. Conventional lead-remediation methods that involve physical removal of lead-contaminated material or site stabilization are expensive, limiting their practical use. As alternatives, researchers have recently explored plant-based bio-remediation technologies. These include applying biochar, a pyrolytic black carbon derived from thermal conversion of plant biomass in an oxygen-limited environment, as a soil additive. Studies have suggested that biochar can efficiently immobilize lead in soil and water, and biochar also tends to have significant positive effects on plant growth due to its ability to improve soil physical properties, nutrient status, pH, and soil organic matter content. Although there have been many studies on the effects of lead toxicity and biochar on soil microbes, the interactive effects of these two factors at low doses of biochar have been difficult to discern due to complex relationships among biochar, plants that reside in the soil, and the soil microbial community. To better understand these interactions, researchers performed a variety of experiments, including some that used the U.S. Department of Energy’s Advanced Photon Source (APS). Their findings, published in the Journal of Hazardous Materials, show that biochar could play a valuable role in shaping healthier soil microbial communities by both sequestering lead through sorption and the formation of insoluble minerals, and serving as microhabitats for various microbial species.

As a model system, the researchers grew banana plants in sandy soil spiked with three different concentrations of lead (Pb): none, 400 mg/kg (the U.S. EPA limit for lead in bare soil of play areas), and 1200 mg/kg (the U.S. EPA standard for lead in non-play areas). To each of these soils, they added concentrations of either 1%, 3%, or 0% biochar derived from pyrolyzing bamboo stems. They worked with 12 pots of each combination of lead and biochar in the soil.

After five months, the researchers took soil samples from three pots for each combination of lead and biochar. They used genetic sequencing to determine which microbial species were living in the soil. They also used the Materials Research Collaborative Access Team (MR-CAT) 10-ID x-ray beamline at the APS to perform x-ray absorption near edge structure (XANES) studies to determine what lead-based compounds had formed in the soil. (The APS is an Office of Science user facility at Argonne National Laboratory.)

Their findings showed that additions of lead or biochar both changed the soil microbe community in different ways. Compared to pristine soil, the addition of lead reduced microbial diversity and increased the population of several classes of bacteria, including Gammaproteobacteria, Aplhaproteobacteria, Verrucomicrobiae, and Acidobacteriia. In contrast, the Bacteroidia population was reduced. The addition of biochar tended to increase Gammaproteobacteria, Bacilli, and Alphaproteobacteria but reduced Bacteroidia and Verrucomicrobiae.

In the pots that mixed lead-contaminated soil with biochar, the biochar appeared to alleviate lead toxicity and increased the diversity of the microbial community, boosting the populations of several species including Alkanibacter, Muciaginibacter, Burkholderiaceae, and Beggiatoaceae.

The researchers suggest a couple of different mechanisms for these effects.

First, the XANES experiments suggest that biochar not only increased sorption of lead by biochar, but also induced the formation of a mineral known as pyromorphite (Pb5(PO4)3Cl/OH), presumably due to biochar’s ability to increase the soil pH and water-holding capacity (Fig. 1). Pyromorphite is several orders of magnitude less soluble than the most commonly found lead-based minerals in soils, such as anglesite and cerussite. In addition, biochar may also serve as a microhabitat niche and could even have been an energy source for microbes, allowing some bacterial species to thrive.

In addition to these positive qualities, biochar also increased the vigor of the banana plants growing in the soil to which it was added. The researchers suggest that this phenomenon may partly be due to the enhanced growth of soil microbes involved in nitrogen cycling.

Together, the researchers suggest, these results support biochar as a potentially effective and inexpensive way to remediate lead contamination in soil while also improving soil microbial health. They propose that further research should focus on gene activity in soil microbes to better understand their response to lead toxicity and their role in nitrogen and carbon cycling.  ― Summer Allen

See: Yongshan Wan1*, Richard Devereux1, S. Elizabeth George1, Jianjun Chen2, Bin Gao2, Matthew Noerpel1, and Kirk Scheckel1, “ Interactive effects of biochar amendment and lead toxicity on soil microbial community,” J. Hazard. Mater. 425, 127921 (2022). DOI:  10.1016/j.jhazmat.2021.127921

Author affiliations:  1 U.S. EPA, 2University of Florida

Correspondence: * wan.yongshan@epa.gov

MR-CAT operations are supported by the Department of Energy and the MRCAT member institutions. All 16S rRNA sequence analyses were performed at the DNA Environmental Sample Preparation and Sequencing Facility of Argonne National Laboratory under an Interagency Agreement between US EPA and DOE Argonne National Laboratory, Biosciences Division DW089924584. This research used resources from the Advanced Photon Source, a U.S. DOE Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357.

The U.S. Department of Energy's APS at Argonne National Laboratory is one of the world’s most productive x-ray light source facilities. Each year, the APS provides high-brightness x-ray beams to a diverse community of more than 5,000 researchers in materials science, chemistry, condensed matter physics, the life and environmental sciences, and applied research. Researchers using the APS produce over 2,000 publications each year detailing impactful discoveries, and solve more vital biological protein structures than users of any other x-ray light source research facility. APS x-rays are ideally suited for explorations of materials and biological structures; elemental distribution; chemical, magnetic, electronic states; and a wide range of technologically important engineering systems from batteries to fuel injector sprays, all of which are the foundations of our nation’s economic, technological, and physical well-being.

Argonne National Laboratory seeks solutions to pressing national problems in science and technology. The nation's first national laboratory, Argonne conducts leading-edge basic and applied scientific research in virtually every scientific discipline. Argonne researchers work closely with researchers from hundreds of companies, universities, and federal, state and municipal agencies to help them solve their specific problems, advance America's scientific leadership and prepare the nation for a better future. With employees from more than 60 nations, Argonne is managed by UChicago Argonne, LLC, for the U.S. DOE Office of Science.

The U.S. Department of Energy's Office of Science is the single largest supporter of basic research in the physical sciences in the United States and is working to address some of the most pressing challenges of our time. For more information, visit the Office of Science website.

 

Published Date
06.20.2022